Cloning of the gene encoding the major outer membrane protein of Haemophilus influenzae type b
نویسندگان
چکیده
منابع مشابه
the investigation of the relationship between type a and type b personalities and quality of translation
چکیده ندارد.
Comparative analysis of the structures of the outer membrane protein P1 genes from major clones of Haemophilus influenzae type b.
P1 outer membrane proteins from Haemophilus influenzae type b are heterogeneous antigenically and with respect to apparent molecular weight in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. For determination of the molecular basis for the differences in the P1 proteins, the genes for the P1 proteins from strain 1613, representative of outer membrane protein subtype 3L, and strain 83...
متن کاملMolecular cloning of DNA coding for outer membrane proteins of Haemophilus influenzae type b.
DNA from Haemophilus influenzae type b was cloned in Escherichia coli with a vector lambda gt11 Amp1. Clones producing antigens reactive with hyperimmune rabbit antisera were identified by colony radioimmune assay. A second screening with hyperimmune serum adsorbed with intact H. influenzae type b bacteria was used to identify those clones producing surface-exposed outer membrane proteins. The ...
متن کاملCharacteristics of major outer membrane proteins of Haemophilus influenzae.
Several properties of Haemophilus influenzae outer membrane proteins were analyzed to define related proteins in various isolates. H. influenzae type b 760705 had six major outer membrane proteins with the following characteristics. Protein a (Mr, 47,000) demonstrated heat modifiability in sodium dodecyl sulfate; its apparent molecular weight was 34,000 at temperatures below 60 degrees C. This ...
متن کاملImmunogenicity and Efficacy of Different Haemophilus influenzae type b Vaccines
Haemophilus influenzae, a major cause of meningitis in young children leading to death and other neurological sequelae. The disease leaves 15 to 35% of the survivors with permanent disabilities, such as, mental retardation or deafness. Despite the availability of new and more powerful antibiotics children with Hib meningitis still suffer from high mortality or morbidity. The emergence of multir...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1988
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.56.10.2709-2716.1988